Brassica napus (also known as oilseed rape) is the third largest oilseed crop in the world, providing 12% of the world’s supply of vegetable oil in 2007. B.napus seed contains approximately 40-45% oil on a dry weight basis. Global characterization of proteins expressed during B. napus seed development will provide a foundation for uncovering temporal relationships between metabolic enzyme expression and oil production.
High resolution two-dimensional gel electrophoresis was used to profile protein expression in seed 2, 3, 4, 5 and 6 weeks after flowering. Two-dimensional gel protein reference maps for proteins with pI values between 4 and 7 (gel pI 4-7) and 7 to 10 (gel pI 3-10) are available as well as a table of protein assignments classified according to function (Protein table). These proteome reference maps contain clickable spots linked to expression profiles and protein identification data.